# How To Calculate Uncertainty Of Pipette

Deliverables: Record of all observations. 2 cm - gives the "absolute" uncertainty. 001, or 10% of the smallest graduation (10% • 0. Modern pipette models have been in use since the late 1950’s. Check that the indicator value is zero, load calibration weight W1 at the center of the pan, and then record the indicator value I1. Let's say you want to find the measurement of the thickness of just one CD case. • Additional uncertainty components depending on the quantity being measured/calibrated All of these above listed uncertainty components are referred as the Type B uncertainty. The MSL Uncertainty Calculator is an Excel spreadsheet that provides an uncertainty table suitable for summarising an uncertainty calculation. According to the provisions in JJG 196-2006, 1 mL pipet’s capacity margin. It would be a reasonable approximation to ignore to pipette uncertainty when calculating the overall uncertainty in the final value. 07%, respectively. We have previously seen in the "Adding & Subtracting: Absolute Uncertainties" video that when a mathematical operation requires addition or subtraction, you. 002 mL, which represents ∼1. :lj"! !:'- * i li Hl!!"tf|. Calculate the volume needed from this concentrated solution to prepare 250 mL of a 10-2M solution. Sample volumes are usually relatively small in titration experiments, so using volumetric pipettes is advised. 06 cm3 Calculate the percentage uncertainty in using the pipette. Search: Accuracy Precision And Percent Error Practice Worksheet Answers. Nominal Display Balance Volume (µL) (mg) Sensitivity < 100 -0. lab report chem pipette calibration post lab introduction the purpose of this laboratory experiment is to exercise pipette calibration and gain familiarity on. In literature number of studies has been published on the evaluation of uncertainty in chemical and physical analysis [ 16 – 19 ] in various matrixes but the evaluation of the. a) 1 ml b) 2 ml c) 5 ml d) 10 ml e) 20 ml f) 25 ml g) 50 ml De-mineralized water is drawn in the pipette and the level of water is accurately adjusted at 0. invert flask to ensure uniform solution - using volumetric pipette more accurate than measure cylinder as smaller uncertainty. 006 mL, while a 50-mL pipette. These are too designed to deliver requested amount of solution and they have a scale on the side. 5-mL pipette has a tolerance of ±0. 002 mL, which represents ∼1. Finally, calculate the expanded uncertainty in y, symbol-ized here with a capital U y , to give a level of confidence for the interval of uncertainty by multiplying the standard uncertainty in Eq. Deliverables: Record of all observations. Pipette performance data are examined to determine whether this degree of trueness can be attained. The design trend is to produce ergonomically designed pipettes which are easy to use and provide less strain on the operator with repetitive use. Calculate the mean volume. 1%, a significant improvement. 02-ml (10% of 0. Mass difference is the mass of water. Modern pipette models have been in use since the late 1950’s. pipette is taken within short period of time. pipets, or burettes are used and on the size of the volume to be measured. 2% of but ∼61% of the much smaller. The uncertainty in a measurement of temperature will be quite different if the. This means that every microliter (µL) should weigh 0. uncertainty due to precision, repeatability, weighing, atomic mass of Pb and air buoyancy. The uncertainty of the pipetted volume u(V) has three main uncertainty components: uncertainty due to repeatability, u(V,rep); uncertainty due to pipette calibration, u(V,cal) and uncertainty due to the temperature difference from 20 °C, u(V,temp). [1 mark] % uncertainty 0 6. A pipette (sometimes spelled pipet) is a laboratory tool commonly used in chemistry, biology and medicine to transport a measured volume of liquid, often as a media dispenser. Since both of these are divisions the relative variance of 1/T is just the relative variance of T (Rule 2). Calculate the percentage uncertainty. Keeping it overly simple can be a non-conformance, and cause serious headaches. Using a 10 mL pipette, obtain approximately 5 mL of the known liquid. 25% We can see that the uncertainly in the pipette measurement is far less than that of either the HCl volume or the time. Quite often single volume pipette and volumetric flask are used together - diluted sample is pipetted from the flask to carry on separate titrations. Deliverables: Record of all observations. 3 Before adding the solution from the burette in the rough titration, there was an air bubble below the tap. Standard uncertainty propagation, not accounting for correlated uncertainties, yields an uncertainty in values of 0. • Introduction to Measurements and Uncertainty - This interactive online tutorial covers measurement and uncertainty as it relates to length, temperature, volume, and exact numbers and also discusses the counting of significant figures. To gain a better understanding of uncertainty, how to take measurements and how to report significant figures properly. 4 Effects of Valve Closure on Pipette Systems. • Additional uncertainty components depending on the quantity being measured/calibrated All of these above listed uncertainty components are referred as the Type B uncertainty. B Volume of 25 cm 3 measured with a measuring cylinder with an uncertainty of ±0. 1%, a significant improvement. 07%, respectively. However, the accuracy of measurements also depends on the quality of the apparatus used (e. Thus, the best solution for pipette checking is application of balances that are not controlled by legal metrology. Deliverables: Record of all observations. 06ml by assuming a rectangular distribution. burette synonyms, burette pronunciation, burette translation, English dictionary definition of burette. 25% We can see that the uncertainly in the pipette measurement is far less than that of either the HCl volume or the time. Absolute Uncertainty (analogue) •Absolute Uncertainty tells you about the resolution of your measuring device and always has the *same units as the measuring device •How to Calculate: •Example 1: How long is the black box in mm with uncertainty? 43 ± 0. This number has the same units as the measured or calculated "best" value. Differences associated with use of different pipettes to dispense the same volume Deterioration of function of a single pipette after calibration Time Run-to-run variation in incubation time Timing of an assay incubation set at the extremes of the manufacturer’s limits Temperature Day-to-day temperature fluctuation of an incubator. In other words, if your pipette is accurate, the amount of water you dispense will equal the amount the water weighs. 006 mL, while a 50-mL pipette. From the histogram, it is clear that the uncertainty in Vp, the volume of the pipette is a major contributor to the total uncertainty, and that, if we want to improve the quality, e. What is the uncertainty of a 25 mL pipette? Making a measurement A 100-ml graduated cylinder with 1-ml graduation will have an uncertainty of +0. It uses the gravimetric method specified in ISO 8655-6 [2] as the reference method for calibrating piston-operated volumetric apparatus. Using a clean pipette, add distilled water to the penny drop by drop until it overflows. Following the incubation, 7. The pipette tips used for mixing and aliquoting the 0°C reactions were kept on ice. So, if your pipette is set to 100 µL, then the scale should. 15/average titre result x100. % uncertainity = !"" !!. 3 - Quantify uncertainty components Measure or estimate the size of the uncertainty component associated with each potential source of uncertainty identified. Calculate the volume needed from this concentrated solution to prepare 250 mL of a 10-2M solution. Although pipettes come in other sizes, you are probably unlikely to come across a bigger one in an A level lab, although you might occasionally use a smaller one such as 5 cm 3. pipette failures occur, and can be determined by tracking a group of pipettes and noting how long it takes for each one to fail. % uncertainity = !"" !!. In other words, if your pipette is accurate, the amount of water you dispense will equal the amount the water weighs. Calculate the accuracy of the pipette. Calculate the density & identify the metal using the posted list. The total uncertainty is calculated based on equation , showing that the total uncertainty in the best cases in u 0 = 30, 40, 50, and 60 m s −1 is 3. Taking the values from the example of the chart above, the formula in Excel would apply as follows for calculating the P90 from P50 with Uncertainty 1 year: Equivalent Hours (P90) = NORMINV(1. 06 cm3) Calculate the percentage uncertainty associated with your average titre (uncertainty associated with each reading of a 50cm3 class B burette is 0. 07%, respectively. Volumetric pipettes usually bear a lesser uncertainty of ± 0. What is the uncertainty of a 25 mL pipette? Making a measurement A 100-ml graduated cylinder with 1-ml graduation will have an uncertainty of +0. Therefore, the uncertainty of volume calibration can be divided into “2-1 Components concerning the mass measurement using a balance,” and “2-2 Components concerning the mass-to-volume conversion. for burette % uncertainty = 0. To gain a better understanding of uncertainty, how to take measurements and how to report significant figures properly. The National Institute of Standards and Technology tolerances for volumetric equipment are given in textbooks on quantitative analysis. 10 mL of the SCN-solution for K c determination into. Calculate the percentage uncertainty associated with the volume of sodium carbonate which you have transferred using a pipette (uncertainty associated with reading a 25cm3 class B pipette is 0. 5-mL pipette has a tolerance of ±0. 06 cm3 Calculate the percentage uncertainty in using the pipette. How to calculate uncertainty of measuring cylinder The uncertainty of the pipetted volume u(V) has three main uncertainty components: uncertainty due to repeatability, u(V,rep); uncertainty due to pipette calibration, u(V,cal) and uncertainty due to the temperature difference from 20 °C, u(V,temp). Chemicals and Materials: Distilled H2O 10 mL Graduated Cylinder 5 mL Pipette 250 mL Beaker 250 mL Flask Scale Solid Sodium Chloride Weigh Boat. …Solution: Don't be put off by multi-step problems, just work one step at a time. for burette % uncertainty = 0. From the histogram, it is clear that the uncertainty in Vp, the volume of the pipette is a major contributor to the total uncertainty, and that, if we want to improve the quality, e. , decrease the uncertainty of the concentration of the working solution, we should focus our attention on Vp. What is the uncertainty of a 25 mL pipette? Making a measurement A 100-ml graduated cylinder with 1-ml graduation will have an uncertainty of +0. First, get the uncertainty in 1/T 2 and 1/T 1. Is uncertainty standard deviation? Standard deviation is the basis of defining standard uncertainty - uncertainty at standard deviation level, denoted by small u. 20x20x180 mm. The standard uncertainty due to the water volume expansion, u v is 0. 005 g Absolute Uncertainty= ½ * smallest increment on measuring device. 01mL with give an uncertainty of + 0. Allometrics offers two types of calibration reports for pipettes. 3 - Quantify uncertainty components Measure or estimate the size of the uncertainty component associated with each potential source of uncertainty identified. You can anchor the pipette in place with your other hand in two ways: either pinch the pipette to the side of the beaker, or grip the stem of the pipette between your index (1st) and middle (2nd) fingers while. Calibrated, adjustable piston pipettes Volumetric glassware Liquid Chromatograph – Tandem Mass Spectrometer All uncertainty components considered: u(r) – experimental uncertainty arising from random effects u(cal) – uncertainty in the preparation of working standard solution used to prepare calibrators. 001, or 10% of the smallest graduation (10% • 0. The percentage uncertainty in the time = 2/32 x 100 = 6. The tolerance depends on the size: a 0. Gravimetric methods are also often recognized as a more economical way of calibration. It uses the gravimetric method specified in ISO 8655-6 [2] as the reference method for calibrating piston-operated volumetric apparatus. This is an example of calculating the volume and its uncertainty of liquid delivered from a self-calibrated volumetric pipette. Find the uncertainty in the result. maximum uncertainty of measurement of 0. Taking the values from the example of the chart above, the formula in Excel would apply as follows for calculating the P90 from P50 with Uncertainty 1 year: Equivalent Hours (P90) = NORMINV(1. Is it OK to use a graduated pipette to take the aliquot? Set this solution aside to determine its exact concentration later. The accuracy is usually measured in terms of the tolerance, which is the uncertainty in a measurement made with the glassware. It lays down the basic first steps required for the program and references the recommended resources for completing the required calculations. Sample volumes are usually relatively small in titration experiments, so using volumetric pipettes is advised. Determine the density of water, at the temperature measured in 6 above, from the tables given. 2 Volumetric Pipettes Calibration of volumetric pipettes of following capacities are performed. lW H, h ,,!!!!i\ If'' «/. What is the uncertainty of a 25 mL pipette? Making a measurement A 100-ml graduated cylinder with 1-ml graduation will have an uncertainty of +0. Use a volumetric pipette to withdraw the aliquot calculated from the concen - trated solution. The labeled RNA concentrations and incubation times are indicated in the individual figure legends. To gain a better understanding of uncertainty, how to take measurements and how to report significant figures properly. [1 mark] % uncertainty 0 6. the axes are labeled appropriately and have units. Adding uncertainties together => combined uncertainty The type A (standard deviation) is something you can calculate, but often some of the various type B uncertainties. • Additional uncertainty components depending on the quantity being measured/calibrated All of these above listed uncertainty components are referred as the Type B uncertainty. a) 1 ml b) 2 ml c) 5 ml d) 10 ml e) 20 ml f) 25 ml g) 50 ml De-mineralized water is drawn in the pipette and the level of water is accurately adjusted at 0. You can anchor the pipette in place with your other hand in two ways: either pinch the pipette to the side of the beaker, or grip the stem of the pipette between your index (1st) and middle (2nd) fingers while. However, the accuracy of measurements also depends on the quality of the apparatus used (e. • Additional uncertainty components depending on the quantity being measured/calibrated All of these above listed uncertainty components are referred as the Type B uncertainty. The percentage uncertainty in the time = 2/32 x 100 = 6. Pipette performance data are examined to determine whether this degree of trueness can be attained. 002 mL, which represents ∼1. Deliverables: Record of all observations. Calculate uncertainty of a single measurement of multiple objects. Using a clean pipette, add distilled water to the penny drop by drop until it overflows. What is the uncertainty of a 25 mL pipette? Making a measurement A 100-ml graduated cylinder with 1-ml graduation will have an uncertainty of +0. Then convert to absolute. Comment on the accuracy of the pipette and show your results to a demonstrator, who will initial the results and make a note of your value. 1 cm3 •burette (s tart & end readings and end point ) 0. This is an example of calculating the volume and its uncertainty of liquid delivered from a self-calibrated volumetric pipette. For a 25-ml graduated cylinder with graduation of 0. Accuracy should be between 99-101%. Use equation 4-27 in your text to determine the uncertainty in the iron concentration in the well water (hint: read pgs 83-91 of your text. In addition to hand operated pipettes, many manufacturers produce semi-automatic electronically controlled pipettes. Re-zero the scale. 20x20x180 mm. uncertainty due to precision, repeatability, weighing, atomic mass of Pb and air buoyancy. Do not forget to start and control the stirring again. For a 25-ml graduated cylinder with graduation of 0. Uncertainty in Measurements • When making measurements, there is always some limitation to how "exact" they can be. The standard uncertainty of a volume measured by a pipette is usually expressed in µl or ml, not nl. This measurement will be so small that your percentage of uncertainty will be a bit high. , decrease the uncertainty of the concentration of the working solution, we should focus our attention on Vp. What is the uncertainty of a 25 mL pipette? Making a measurement A 100-ml graduated cylinder with 1-ml graduation will have an uncertainty of +0. (9) by the 95% coverage factor, or Student t-statistic: U y =±t 95%, v y u y (11) We use the conventional 95% coverage, but are certainly free to. Calculate the accuracy of the pipette. Let's say you're measuring a stack of 10 CD cases that are all the same length. Which of these pieces of apparatus has the lowest percentage uncertainty in the measurement shown? A Volume of 25 cm 3 measured with a burette with an uncertainty of ±0. Use equation 4-27 in your text to determine the uncertainty in the iron concentration in the well water (hint: read pgs 83-91 of your text. Following the incubation, 7. This Technical Report gives the detailed evaluation of uncertainty for volume measurements according to the Guide to the Expression of Uncertainty in Measurement (GUM). 06ml by assuming a rectangular distribution. 08 Note that this manner of calculation applies only on uncertainty components with. This measurement will be so small that your percentage of uncertainty will be a bit high. :lj"! !:'- * i li Hl!!"tf|. Calculate the concentration of iron in the well water sample from the equation of the calibration line determined using Excel. The most common way to check your pipette accuracy is by weighing water. This pipette, when used at its full scale setting, can be tested against a 2% tolerance. Finally, calculate the expanded uncertainty in y, symbol-ized here with a capital U y , to give a level of confidence for the interval of uncertainty by multiplying the standard uncertainty in Eq. pipette is measured using a balance, and the measured mass value is then converted to a volumetric value. Deliverables: Record of all observations. , decrease the uncertainty of the concentration of the working solution, we should focus our attention on Vp. maximum uncertainty of measurement of 0. To calculate the accuracy you can use the equation A = 100 x V avg /V 0, where A is the accuracy of the pipette, V avg is the average calculated volume and V 0 is the value you set the pipette to dispense. Mass difference is the mass of water. Many scientific studies involve either dilutions made with pipettes, or the addition of very small volumes of liquid to an experiment. Comment on the accuracy of the pipette and show your results to a demonstrator, who will initial the results and make a note of your value. Volumetric pipettes are commonly used in analytical chemistry to make laboratory solutions from a base stock as well as to prepare solutions for titration. These are too designed to deliver requested amount of solution and they have a scale on the side. i!iif|i liiii KM (,,- ,11 I I! !!!!»!I'm :;. To gain a better understanding of uncertainty, how to take measurements and how to report significant figures properly. Read and record the liquid volume from the marks on the pipette, using the correct number of significant figures. Finally, calculate the expanded uncertainty in y, symbol-ized here with a capital U y , to give a level of confidence for the interval of uncertainty by multiplying the standard uncertainty in Eq. It would be a reasonable approximation to ignore to pipette uncertainty when calculating the overall uncertainty in the final value. In other words, if your pipette is accurate, the amount of water you dispense will equal the amount the water weighs. 25% We can see that the uncertainly in the pipette measurement is far less than that of either the HCl volume or the time. It uses the gravimetric method specified in ISO 8655-6 [2] as the reference method for calibrating piston-operated volumetric apparatus. For a 25-ml graduated cylinder with graduation of 0. 006 mL, while a 50-mL pipette. [1 mark] % uncertainty 0 6. According to the provisions in JJG 196-2006, 1 mL pipet’s capacity margin. How to calculate uncertainty of measuring cylinder The uncertainty of the pipetted volume u(V) has three main uncertainty components: uncertainty due to repeatability, u(V,rep); uncertainty due to pipette calibration, u(V,cal) and uncertainty due to the temperature difference from 20 °C, u(V,temp). 2 cm - gives the "absolute" uncertainty. With a new and rinsed pipette tip, pipette 2. So, if your pipette is set to 100 µL, then the scale should. 150 g measured with a balance with an uncertainty of ±0. The National Physical Laboratory is operated on behalf of the DTI by NPL Management Limited, a wholly owned subsidiary of Serco Group plc The use and calibration of piston-operated volumetric pipettes Measurement Good Practice Guide. Be sure to count the drops! Record the number of drops for Trial 1 in Data Table 1 below. Deliverables: Record of all observations. The density of water is 1 g/mL. Repeat 3-4 times. Then convert to absolute. Volumetric pipets, flasks and burets are the most accurate; the glassware makers calibrate these to a high level of accuracy. You can anchor the pipette in place with your other hand in two ways: either pinch the pipette to the side of the beaker, or grip the stem of the pipette between your index (1st) and middle (2nd) fingers while. How to calculate uncertainty of measuring cylinder The uncertainty of the pipetted volume u(V) has three main uncertainty components: uncertainty due to repeatability, u(V,rep); uncertainty due to pipette calibration, u(V,cal) and uncertainty due to the temperature difference from 20 °C, u(V,temp). Pipettes are a laboratory tool used mostly in chemistry, biology and medicine. Calculate the percentage uncertainty associated with the volume of sodium carbonate which you have transferred using a pipette (uncertainty associated with reading a 25cm3 class B pipette is 0. 150 g measured with a balance with an uncertainty of ±0. According to the provisions in JJG 196-2006, 1 mL pipet’s capacity margin. Following the incubation, 7. 2 Volumetric Pipettes Calibration of volumetric pipettes of following capacities are performed. the axes are labeled appropriately and have units. i!iif|i liiii KM (,,- ,11 I I! !!!!»!I'm :;. 01mL with give an uncertainty of + 0. The procedure provides a flowchart and defines the steps required to plan, measure and calculate the data required for an evaluation of measurement uncertainty program. The tolerance depends on the size: a 0. There are several different models of pipettes used for different methods. :lj"! !:'- * i li Hl!!"tf|. 26 ml @ 20ºC. Be sure to add a new layer of soap between trials. To gain a better understanding of uncertainty, how to take measurements and how to report significant figures properly. This is an example of calculating the volume and its uncertainty of liquid delivered from a self-calibrated volumetric pipette. By considering. Graduated cylinders generally have a measured volume uncertainty of 0. The MSL Uncertainty Calculator is an Excel spreadsheet that provides an uncertainty table suitable for summarising an uncertainty calculation. With a new and rinsed pipette tip, pipette 2. lab report chem pipette calibration post lab introduction the purpose of this laboratory experiment is to exercise pipette calibration and gain familiarity on. Is uncertainty standard deviation? Standard deviation is the basis of defining standard uncertainty - uncertainty at standard deviation level, denoted by small u. The uncertainty in a measurement of temperature will be quite different if the. 26 ml and - 0. Calculate the relative uncertainty using the mean deviation as the absolute uncertainty and the mean as a typical measurement. % uncertainity = !"" !!. Pipettes are used in regulated medical testing. Two other types of volumetric glass are graduated pipettes and graduated cylinders. Having regard to above, it is convenient that balances used for pipettes checking have possibility of adjusting parameters of automatic internal time and temperature calibration. If comparative mean differences are consistently less than 1/3 of the accuracy specification (min. An extensive range of commercially made pipettes is currently available (Figure 3). 0107/√3 or 0. maximum uncertainty of measurement of 0. A small dry, empty beaker is placed on the balance and its tare weight (W0) is recorded. 3ml @ 20 °C. According to the provisions in JJG 196-2006, 1 mL pipet’s capacity margin. They’re used to measure or dispense a specific volume of liquid. 08 Note that this manner of calculation applies only on uncertainty components with. Mass difference is the mass of water. Many scientific studies involve either dilutions made with pipettes, or the addition of very small volumes of liquid to an experiment. Relative uncertainty: this is sometimes called the fractional uncertainty and we often express it. With a new and rinsed pipette tip, pipette 0. 2 K calculate (1/T 2 - 1/T 1). This pipette, when used at its full scale setting, can be tested against a 2% tolerance. They come in two types: volumetric and measuring pipettes. This means that when the pipette is used exactly as per the manufacturer's instructions at 20ºC there is an in-built inaccuracy of between + 0. This measurement will be so small that your percentage of uncertainty will be a bit high. Modern pipette models have been in use since the late 1950’s. To gain a better understanding of uncertainty, how to take measurements and how to report significant figures properly. Nominal Display Balance Volume (µL) (mg) Sensitivity < 100 -0. The uncertainty of the pipetted volume u(V) has three main uncertainty components: uncertainty due to repeatability, u(V,rep); uncertainty due to pipette calibration, u(V,cal) and uncertainty due to the temperature difference from 20 °C, u(V,temp). Example: Calculate the percentage uncertainty when 24. Therefore, the combined standard uncertainty of this 10-ml pipette is 𝑢𝑝2=√𝑢𝑚2+𝑢𝑟2+𝑢 2=√0. There are sections on moving decimal points, counting zeros, and rounding. Burette is similar to the pipette, as it is designed to measure volume of the delivered liquid, but it can measure any volume of the solution. (9) by the 95% coverage factor, or Student t-statistic: U y =±t 95%, v y u y (11) We use the conventional 95% coverage, but are certainly free to. Read and record the liquid volume from the marks on the pipette, using the correct number of significant figures. To gain a better understanding of uncertainty, how to take measurements and how to report significant figures properly. This measurement will be so small that your percentage of uncertainty will be a bit high. An extensive range of commercially made pipettes is currently available (Figure 3). The percentage uncertainty in the time = 2/32 x 100 = 6. Calculate the percentage uncertainty associated with the volume of sodium carbonate which you have transferred using a pipette (uncertainty associated with reading a 25cm3 class B pipette is 0. The simplest and most reliable solution is to replace the 200 μL pipette with a 100 μL pipette. It is applicable to piston pipettes, piston burettes, dilutors and dispensers. What is the uncertainty of a 25 mL pipette? Making a measurement A 100-ml graduated cylinder with 1-ml graduation will have an uncertainty of +0. 40% e The balance used to measure out the acid had an uncertainty of ±0. The standard uncertainty of a volume measured by a pipette is usually expressed in µl or ml, not nl. i!iif|i liiii KM (,,- ,11 I I! !!!!»!I'm :;. The most common sizes are 25 cm 3 and 10 cm 3. 2 ml, the uncertainty is +. However, the accuracy of measurements also depends on the quality of the apparatus used (e. 2 cm - gives the "absolute" uncertainty. 2 ml are delivered from a burette. Calculate the density & identify the metal using the posted list. 4 Effects of Valve Closure on Pipette Systems. A 1-ml pipette with a graduation of 0. Many scientific studies involve either dilutions made with pipettes, or the addition of very small volumes of liquid to an experiment. To calculate the accuracy you can use the equation A = 100 x V avg /V 0, where A is the accuracy of the pipette, V avg is the average calculated volume and V 0 is the value you set the pipette to dispense. Do not forget to start and control the stirring again. Calculate the percentage uncertainty for weighing out this 1. a) 1 ml b) 2 ml c) 5 ml d) 10 ml e) 20 ml f) 25 ml g) 50 ml De-mineralized water is drawn in the pipette and the level of water is accurately adjusted at 0. Quite often single volume pipette and volumetric flask are used together - diluted sample is pipetted from the flask to carry on separate titrations. 2% of but ∼61% of the much smaller. % uncertainity = !"" !!. In addition to hand operated pipettes, many manufacturers produce semi-automatic electronically controlled pipettes. Deliverables: Record of all observations. 10 mL of the SCN-solution for K c determination into. Pipettes are used in regulated medical testing. The relative uncertainty gives the uncertainty as a percentage of the original value. •25 cm3 pipette 0. Be sure to count the drops! Record the number of drops for Trial 1 in Data Table 1 below. 2 ml, the uncertainty is +. pipette failures occur, and can be determined by tracking a group of pipettes and noting how long it takes for each one to fail. Is uncertainty standard deviation? Standard deviation is the basis of defining standard uncertainty - uncertainty at standard deviation level, denoted by small u. make up to mark with distilled water and dropping pipette 3. It uses the gravimetric method specified in ISO 8655-6 [2] as the reference method for calibrating piston-operated volumetric apparatus. 2 cm - gives the "absolute" uncertainty. With a new and rinsed pipette tip, pipette 0. Pipette has been tested, according to the calibration certificate, 1 mL single standard pipette’s actual capacity is 0. It lays down the basic first steps required for the program and references the recommended resources for completing the required calculations. From the histogram, it is clear that the uncertainty in Vp, the volume of the pipette is a major contributor to the total uncertainty, and that, if we want to improve the quality, e. The uncertainty of the pipetted volume u(V) has three main uncertainty components: uncertainty due to repeatability, u(V,rep); uncertainty due to pipette calibration, u(V,cal) and uncertainty due to the temperature difference from 20 °C, u(V,temp). The National Institute of Standards and Technology tolerances for volumetric equipment are given in textbooks on quantitative analysis. Burette is similar to the pipette, as it is designed to measure volume of the delivered liquid, but it can measure any volume of the solution. 3 - Quantify uncertainty components Measure or estimate the size of the uncertainty component associated with each potential source of uncertainty identified. Figure 13 shows the skin-friction values and their total uncertainties that satisfy —measurements when the average oil-film thickness is close to the. 08 Note that this manner of calculation applies only on uncertainty components with. The estimate of the probable maximum difference of the pipette volume from the nominal volume,. To gain a better understanding of uncertainty, how to take measurements and how to report significant figures properly. Uncertainty Components in Volume Calibration by Gravimetric Method In the gravimetric method, the mass value of the distilled water dispensed from a capacity meter such as a pipette is measured using a balance, and the measured mass value is then converted to a volumetric value. Many scientific studies involve either dilutions made with pipettes, or the addition of very small volumes of liquid to an experiment. 2 Volumetric Pipettes Calibration of volumetric pipettes of following capacities are performed. Note: These requirements are more rigorous than those specified in ISO 8655-6, Table 1. The accuracy is usually measured in terms of the tolerance, which is the uncertainty in a measurement made with the glassware. The labeled RNA concentrations and incubation times are indicated in the individual figure legends. Pipettes are used to measure out fixed volumes of liquids very accurately. The standard uncertainty of a volume measured by a pipette is usually expressed in µl or ml, not nl. burette synonyms, burette pronunciation, burette translation, English dictionary definition of burette. This is an example of calculating the volume and its uncertainty of liquid delivered from a self-calibrated volumetric pipette. An extensive range of commercially made pipettes is currently available (Figure 3). Standard uncertainty propagation, not accounting for correlated uncertainties, yields an uncertainty in values of 0. 15/average titre result x100. 2 K calculate (1/T 2 - 1/T 1). The standard uncertainty of a volume measured by a pipette is usually expressed in µl or ml, not nl. C Mass of 0. This pipette, when used at its full scale setting, can be tested against a 2% tolerance. Determine the density of water, at the temperature measured in 6 above, from the tables given. According to the provisions in JJG 196-2006, 1 mL pipet’s capacity margin. 26 ml @ 20ºC. It is located to the right of the "±". B Volume of 25 cm 3 measured with a measuring cylinder with an uncertainty of ±0. Deliverables: Record of all observations. Calculate the concentration of iron in the well water sample from the equation of the calibration line determined using Excel. Uncertainty in Measurements • When making measurements, there is always some limitation to how "exact" they can be. The uncertainty in a measurement of temperature will be quite different if the. It lays down the basic first steps required for the program and references the recommended resources for completing the required calculations. Therefore, the combined standard uncertainty of this 10-ml pipette is 𝑢𝑝2=√𝑢𝑚2+𝑢𝑟2+𝑢 2=√0. Any undesired variations in these quantities can jeopardise results and so it is essential to take all steps necessary to maximise pipetting accuracy and precision and reduce the level of uncertainty. 0107/√3 or 0. Calculate the volume needed from this concentrated solution to prepare 250 mL of a 10-2M solution. Using a 10 mL pipette, obtain approximately 5 mL of the known liquid. 4 Effects of Valve Closure on Pipette Systems. Determine and record the mass of the liquid using the digital scale. It uses the gravimetric method specified in ISO 8655-6 [2] as the reference method for calibrating piston-operated volumetric apparatus. 15 cm3 Calculate the percentage error for each piece of equipment used by % uncertainty = uncertainty x 100 Measurement made on apparatus e. This measurement will be so small that your percentage of uncertainty will be a bit high. Let's say you want to find the measurement of the thickness of just one CD case. 1 cm3 •burette (s tart & end readings and end point ) 0. In other words, if your pipette is accurate, the amount of water you dispense will equal the amount the water weighs. invert flask to ensure uniform solution - using volumetric pipette more accurate than measure cylinder as smaller uncertainty. The pipette tips used for mixing and aliquoting the 0°C reactions were kept on ice. • Additional uncertainty components depending on the quantity being measured/calibrated All of these above listed uncertainty components are referred as the Type B uncertainty. The uncertainty of the pipetted volume u ( V) has three main uncertainty components: uncertainty due to repeatability, u ( V ,rep); uncertainty due to pipette calibration, u ( V ,cal) and uncertainty due to the temperature difference from 20 °C, u ( V ,temp). pipette 25cm3 of original solution into a 250cm3 volumetric flask 2. A pipette (sometimes spelled pipet) is a laboratory tool commonly used in chemistry, biology and medicine to transport a measured volume of liquid, often as a media dispenser. Calculate the volume needed from this concentrated solution to prepare 250 mL of a 10-2M solution. d The pipette used to measure out the acid into the conical flask had an uncertainty of ±0. It also gives user recommendations. The simplest and most reliable solution is to replace the 200 μL pipette with a 100 μL pipette. Is uncertainty standard deviation? Standard deviation is the basis of defining standard uncertainty - uncertainty at standard deviation level, denoted by small u. Calculate the percentage uncertainty. i!iif|i liiii KM (,,- ,11 I I! !!!!»!I'm :;. 3 consecutive pipettes), continue to the next trial. The standard uncertainty due to the water volume expansion, u v is 0. 06 cm3 Calculate the percentage uncertainty in using the pipette. ASTM standard E969 defines the standard tolerance for volumetric transfer pipettes. Many scientific studies involve either dilutions made with pipettes, or the addition of very small volumes of liquid to an experiment. Is it OK to use a graduated pipette to take the aliquot? Set this solution aside to determine its exact concentration later. To gain a better understanding of uncertainty, how to take measurements and how to report significant figures properly. 2 cm - gives the "absolute" uncertainty. Absolute uncertainty: this is the largest likely difference between the best estimate and the true value. 4 - Calculate combined uncertainty The information obtained in step 3 will consist of a number of quantified contributions to overall uncertainty, whether associated with individual. 15/average titre result x100. pipette 25cm3 of original solution into a 250cm3 volumetric flask 2. The other substance is placed in the burette burette pipette Calculate the number of moles of sodium hydroxide used To calculate the maximum total percentage apparatus uncertainty in the final result add all the individual equipment uncertainties together. [1 mark] % uncertainty 0 6. Repeat steps 4-6 for a total of five trials. 25% We can see that the uncertainly in the pipette measurement is far less than that of either the HCl volume or the time. The standard uncertainty of a volume measured by a pipette is usually expressed in µl or ml, not nl. 3 consecutive pipettes), continue to the next trial. invert flask to ensure uniform solution - using volumetric pipette more accurate than measure cylinder as smaller uncertainty. The percentage uncertainty in the time = 2/32 x 100 = 6. 26 ml and - 0. Calculate the concentration of iron in the well water sample from the equation of the calibration line determined using Excel. Do not forget to start and control the stirring again. 3 - Quantify uncertainty components Measure or estimate the size of the uncertainty component associated with each potential source of uncertainty identified. The tolerance depends on the size: a 0. 06ml by assuming a rectangular distribution. Therefore, the combined standard uncertainty of this 10-ml pipette is 𝑢𝑝2=√𝑢𝑚2+𝑢𝑟2+𝑢 2=√0. It would be a reasonable approximation to ignore to pipette uncertainty when calculating the overall uncertainty in the final value. Differences associated with use of different pipettes to dispense the same volume Deterioration of function of a single pipette after calibration Time Run-to-run variation in incubation time Timing of an assay incubation set at the extremes of the manufacturer’s limits Temperature Day-to-day temperature fluctuation of an incubator. 00 mL of the Fe3+ solution for K c determination into cuvette and place the cuvette into the spectrometer. This measurement will be so small that your percentage of uncertainty will be a bit high. for burette % uncertainty = 0. a) 1 ml b) 2 ml c) 5 ml d) 10 ml e) 20 ml f) 25 ml g) 50 ml De-mineralized water is drawn in the pipette and the level of water is accurately adjusted at 0. 2% of but ∼61% of the much smaller. The simplest and most reliable solution is to replace the 200 μL pipette with a 100 μL pipette. The procedure provides a flowchart and defines the steps required to plan, measure and calculate the data required for an evaluation of measurement uncertainty program. Use equation 4-27 in your text to determine the uncertainty in the iron concentration in the well water (hint: read pgs 83-91 of your text. Pipette has been tested, according to the calibration certificate, 1 mL single standard pipette’s actual capacity is 0. Relative uncertainty: this is sometimes called the fractional uncertainty and we often express it. The standard uncertainty of a volume measured by a pipette is usually expressed in µl or ml, not nl. pipette 25cm3 of original solution into a 250cm3 volumetric flask 2. lab report chem pipette calibration post lab introduction the purpose of this laboratory experiment is to exercise pipette calibration and gain familiarity on. To gain a better understanding of uncertainty, how to take measurements and how to report significant figures properly. The estimate of the probable maximum difference of the pipette volume from the nominal volume,. Two other types of volumetric glass are graduated pipettes and graduated cylinders. According to the provisions in JJG 196-2006, 1 mL pipet’s capacity margin. The National Institute of Standards and Technology tolerances for volumetric equipment are given in textbooks on quantitative analysis. • Additional uncertainty components depending on the quantity being measured/calibrated All of these above listed uncertainty components are referred as the Type B uncertainty. The uncertainty of the pipetted volume u ( V) has three main uncertainty components: uncertainty due to repeatability, u ( V ,rep); uncertainty due to pipette calibration, u ( V ,cal) and uncertainty due to the temperature difference from 20 °C, u ( V ,temp). Deliverables: Record of all observations. Calculate the concentration of iron in the well water sample from the equation of the calibration line determined using Excel. lW H, h ,,!!!!i\ If'' «/. Deliverables: Record of all observations. above, lower the pipette into the beaker so that the tip of the pipette is position well below the surface of the liquid. Differences associated with use of different pipettes to dispense the same volume Deterioration of function of a single pipette after calibration Time Run-to-run variation in incubation time Timing of an assay incubation set at the extremes of the manufacturer’s limits Temperature Day-to-day temperature fluctuation of an incubator. Pipettes are used in regulated medical testing. 10 mL of the SCN-solution for K c determination into. 3ml @ 20 °C. It is located to the right of the "±". Pipette has been tested, according to the calibration certificate, 1 mL single standard pipette’s actual capacity is 0. 3 consecutive pipettes), continue to the next trial. Absolute uncertainty: this is the largest likely difference between the best estimate and the true value. This means that when the pipette is used exactly as per the manufacturer's instructions at 20ºC there is an in-built inaccuracy of between + 0. Mass difference is the mass of water. The National Physical Laboratory is operated on behalf of the DTI by NPL Management Limited, a wholly owned subsidiary of Serco Group plc The use and calibration of piston-operated volumetric pipettes Measurement Good Practice Guide. The uncertainty in using the pipette in this experiment is ±0. The pipette may have the tolerance written on the side as: ± 0. 2 ml, the uncertainty is +. i!iif|i liiii KM (,,- ,11 I I! !!!!»!I'm :;. The National Physical Laboratory is operated on behalf of the DTI by NPL Management Limited, a wholly owned subsidiary of Serco Group plc The use and calibration of piston-operated volumetric pipettes Measurement Good Practice Guide. It is located to the right of the "±". Calculate the percentage uncertainty. uncertainty due to precision, repeatability, weighing, atomic mass of Pb and air buoyancy. pipette failures occur, and can be determined by tracking a group of pipettes and noting how long it takes for each one to fail. 25% We can see that the uncertainly in the pipette measurement is far less than that of either the HCl volume or the time. Volumetric pipettes usually bear a lesser uncertainty of ± 0. Use a volumetric pipette to withdraw the aliquot calculated from the concen - trated solution. 1%, a significant improvement. To gain a better understanding of uncertainty, how to take measurements and how to report significant figures properly. Two other types of volumetric glass are graduated pipettes and graduated cylinders. Calculate the concentration of iron in the well water sample from the equation of the calibration line determined using Excel. An extensive range of commercially made pipettes is currently available (Figure 3). Sample volumes are usually relatively small in titration experiments, so using volumetric pipettes is advised. The pipette tips used for mixing and aliquoting the 0°C reactions were kept on ice. The uncertainty of the pipetted volume u(V) has three main uncertainty components: uncertainty due to repeatability, u(V,rep); uncertainty due to pipette calibration, u(V,cal) and uncertainty due to the temperature difference from 20 °C, u(V,temp). Chemicals and Materials: Distilled H2O 10 mL Graduated Cylinder 5 mL Pipette 250 mL Beaker 250 mL Flask Scale Solid Sodium Chloride Weigh Boat. The pipette may have the tolerance written on the side as: ± 0. Determine and record the mass of the liquid using the digital scale. Taking the values from the example of the chart above, the formula in Excel would apply as follows for calculating the P90 from P50 with Uncertainty 1 year: Equivalent Hours (P90) = NORMINV(1. maximum uncertainty of measurement of 0. 08 Note that this manner of calculation applies only on uncertainty components with. ASTM standard E969 defines the standard tolerance for volumetric transfer pipettes. Accuracy should be between 99-101%. Deliverables: Record of all observations. Calculate the percentage uncertainty for weighing out this 1. We have previously seen in the "Adding & Subtracting: Absolute Uncertainties" video that when a mathematical operation requires addition or subtraction, you. Pipettes are used to measure out fixed volumes of liquids very accurately. 2 K calculate (1/T 2 - 1/T 1). In addition to hand operated pipettes, many manufacturers produce semi-automatic electronically controlled pipettes. Reliability The opposite of uncertainty; high uncertainty = not very reliable measurement Accuracy of measurements Uncertainty is often taken to be half a division on either side of the smallest unit on the scale. 2 cm - gives the "absolute" uncertainty. The percentage uncertainty in the time = 2/32 x 100 = 6. Pipettes are used in regulated medical testing. Therefore, the uncertainty of volume calibration can be divided into “2-1 Components concerning the mass measurement using a balance,” and “2-2 Components concerning the mass-to-volume conversion. The relative uncertainty gives the uncertainty as a percentage of the original value. Calculate the relative uncertainty using the mean deviation as the absolute uncertainty and the mean as a typical measurement. pipette is measured using a balance, and the measured mass value is then converted to a volumetric value. It also gives user recommendations. Deliverables: Record of all observations. Sample volumes are usually relatively small in titration experiments, so using volumetric pipettes is advised. To calculate the accuracy you can use the equation A = 100 x V avg /V 0, where A is the accuracy of the pipette, V avg is the average calculated volume and V 0 is the value you set the pipette to dispense. The percentage uncertainty in the time = 2/32 x 100 = 6. 00 mL of the Fe3+ solution for K c determination into cuvette and place the cuvette into the spectrometer. 25% Ficoll PM 400 (Sigma-Aldrich, Saint Louis, MO), 0. Determine the density of water, at the temperature measured in 6 above, from the tables given. 3ml @ 20 °C. Two other types of volumetric glass are graduated pipettes and graduated cylinders. However, the accuracy of measurements also depends on the quality of the apparatus used (e. A pipette (sometimes spelled pipet) is a laboratory tool commonly used in chemistry, biology and medicine to transport a measured volume of liquid, often as a media dispenser. It uses the gravimetric method specified in ISO 8655-6 [2] as the reference method for calibrating piston-operated volumetric apparatus. Keeping it overly simple can be a non-conformance, and cause serious headaches. Example: Calculate the percentage uncertainty when 24. 06ml by assuming a rectangular distribution. Finally, calculate the expanded uncertainty in y, symbol-ized here with a capital U y , to give a level of confidence for the interval of uncertainty by multiplying the standard uncertainty in Eq. Pipettes are a laboratory tool used mostly in chemistry, biology and medicine. 15 cm3 Calculate the percentage error for each piece of equipment used by % uncertainty = uncertainty x 100 Measurement made on apparatus e. Chemicals and Materials: Distilled H2O 10 mL Graduated Cylinder 5 mL Pipette 250 mL Beaker 250 mL Flask Scale Solid Sodium Chloride Weigh Boat. 3ml @ 20 °C. Absolute uncertainty: this is the largest likely difference between the best estimate and the true value. Taking the values from the example of the chart above, the formula in Excel would apply as follows for calculating the P90 from P50 with Uncertainty 1 year: Equivalent Hours (P90) = NORMINV(1. The estimate of the probable maximum difference of the pipette volume from the nominal volume, expressed as ±x is often used as the estimate of calibration uncertainty of the pipette (as was done in section 4. It furthermore defines terms for the use of piston-operated volumetric apparatus and gives a list of equivalent terms. To gain a better understanding of uncertainty, how to take measurements and how to report significant figures properly. This is an example of calculating the volume and its uncertainty of liquid delivered from a self-calibrated volumetric pipette. How to calculate uncertainty of measuring cylinder The uncertainty of the pipetted volume u(V) has three main uncertainty components: uncertainty due to repeatability, u(V,rep); uncertainty due to pipette calibration, u(V,cal) and uncertainty due to the temperature difference from 20 °C, u(V,temp). Although pipettes come in other sizes, you are probably unlikely to come across a bigger one in an A level lab, although you might occasionally use a smaller one such as 5 cm 3. With a new and rinsed pipette tip, pipette 2. With a new and rinsed pipette tip, pipette 0. Calculate the density & identify the metal using the posted list. Calculate the mean volume. 06ml by assuming a rectangular distribution. Re-zero the scale. …Solution: Don't be put off by multi-step problems, just work one step at a time. Chemicals and Materials: Distilled H2O 10 mL Graduated Cylinder 5 mL Pipette 250 mL Beaker 250 mL Flask Scale Solid Sodium Chloride Weigh Boat. Pipette water into the bottle, close, weigh again. This Technical Report gives the detailed evaluation of uncertainty for volume measurements according to the Guide to the Expression of Uncertainty in Measurement (GUM). Therefore, the combined standard uncertainty of this 10-ml pipette is 𝑢𝑝2=√𝑢𝑚2+𝑢𝑟2+𝑢 2=√0. :lj"! !:'- * i li Hl!!"tf|. Deliverables: Record of all observations. What is the uncertainty of a 25 mL pipette? Making a measurement A 100-ml graduated cylinder with 1-ml graduation will have an uncertainty of +0. According to the provisions in JJG 196-2006, 1 mL pipet’s capacity margin. The standard uncertainty of a volume measured by a pipette is usually expressed in µl or ml, not nl. lW H, h ,,!!!!i\ If'' «/. Any undesired variations in these quantities can jeopardise results and so it is essential to take all steps necessary to maximise pipetting accuracy and precision and reduce the level of uncertainty. above, lower the pipette into the beaker so that the tip of the pipette is position well below the surface of the liquid. Use a volumetric pipette to withdraw the aliquot calculated from the concen - trated solution. This number has the same units as the measured or calculated "best" value. In addition to hand operated pipettes, many manufacturers produce semi-automatic electronically controlled pipettes. 06 cm3) Calculate the percentage uncertainty associated with your average titre (uncertainty associated with each reading of a 50cm3 class B burette is 0. How to calculate uncertainty of measuring cylinder The uncertainty of the pipetted volume u(V) has three main uncertainty components: uncertainty due to repeatability, u(V,rep); uncertainty due to pipette calibration, u(V,cal) and uncertainty due to the temperature difference from 20 °C, u(V,temp). In other words, it explicitly tells you the amount by which the original measurement could be incorrect. Adding uncertainties together => combined uncertainty The type A (standard deviation) is something you can calculate, but often some of the various type B uncertainties. With a new and rinsed pipette tip, pipette 0. Let's say you're measuring a stack of 10 CD cases that are all the same length. pipette 25cm3 of original solution into a 250cm3 volumetric flask 2. • Additional uncertainty components depending on the quantity being measured/calibrated All of these above listed uncertainty components are referred as the Type B uncertainty. , decrease the uncertainty of the concentration of the working solution, we should focus our attention on Vp. % uncertainity = !"" !!. The National Institute of Standards and Technology tolerances for volumetric equipment are given in textbooks on quantitative analysis. Do not forget to start and control the stirring again. 2 ml are delivered from a burette. 1%, a significant improvement. A small dry, empty beaker is placed on the balance and its tare weight (W0) is recorded. lab report chem pipette calibration post lab introduction the purpose of this laboratory experiment is to exercise pipette calibration and gain familiarity on. 2 cm - gives the "absolute" uncertainty. 2 Volumetric Pipettes Calibration of volumetric pipettes of following capacities are performed. So, if your pipette is set to 100 µL, then the scale should. 07%, respectively. Pipette has been tested, according to the calibration certificate, 1 mL single standard pipette’s actual capacity is 0. However, the accuracy of measurements also depends on the quality of the apparatus used (e. The uncertainty of the pipetted volume u(V) has three main uncertainty components: uncertainty due to repeatability, u(V,rep); uncertainty due to pipette calibration, u(V,cal) and uncertainty due to the temperature difference from 20 °C, u(V,temp). In this video I explain how to calculate the uncertainty or error associated with using a burette, volumetric pipette, volumetric flask and balance, how to r. First, get the uncertainty in 1/T 2 and 1/T 1. It is applicable to piston pipettes, piston burettes, dilutors and dispensers. You can anchor the pipette in place with your other hand in two ways: either pinch the pipette to the side of the beaker, or grip the stem of the pipette between your index (1st) and middle (2nd) fingers while. To gain a better understanding of uncertainty, how to take measurements and how to report significant figures properly. In the calibration of a Pipette, the uncertainty calculations should to be performed in an accepted manner. 20x20x180 mm. Step 4: Calculate the total uncertainty (combined standard uncertainty) The manufacturer’s specification for the pipette is: 10. There are sections on moving decimal points, counting zeros, and rounding. First, get the uncertainty in 1/T 2 and 1/T 1. 07%, respectively. pipette is taken within short period of time. Pipettes are used in regulated medical testing. Calculate the mean volume. If comparative mean differences are consistently less than 1/3 of the accuracy specification (min. 3 - Quantify uncertainty components Measure or estimate the size of the uncertainty component associated with each potential source of uncertainty identified. Pipette performance data are examined to determine whether this degree of trueness can be attained. In literature number of studies has been published on the evaluation of uncertainty in chemical and physical analysis [ 16 – 19 ] in various matrixes but the evaluation of the. Absolute Uncertainty (analogue) •Absolute Uncertainty tells you about the resolution of your measuring device and always has the *same units as the measuring device •How to Calculate: •Example 1: How long is the black box in mm with uncertainty? 43 ± 0. Although pipettes come in other sizes, you are probably unlikely to come across a bigger one in an A level lab, although you might occasionally use a smaller one such as 5 cm 3. make up to mark with distilled water and dropping pipette 3. This number has the same units as the measured or calculated "best" value. The first is ISO/IEC-17025 which conforms to tolerances, calibration equipment, calibration test points and uncertainty calculations specified by ISO 8655 and ISO/IEC-17025. Chemicals and Materials: Distilled H2O 10 mL Graduated Cylinder 5 mL Pipette 250 mL Beaker 250 mL Flask Scale Solid Sodium Chloride Weigh Boat. Which of these pieces of apparatus has the lowest percentage uncertainty in the measurement shown? A Volume of 25 cm 3 measured with a burette with an uncertainty of ±0.